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OptiMaix scaffolds are highly purified and can therefore be seeded in the dry state without a previous washing step (preferred method). OptiMaix-3D scaffolds are characterized by a rapid and effective uptake of the cell suspension due to the capillary action of the 3D-structure. Two procedures can be used for seeding the scaffolds with cells:
The “Drop-on” cell seeding method allows control over the exact number of cells seeded on the scaffold. During the “Drop-on” method a defined volume of the cell suspension is pipetted onto one side of the scaffold to achieve near-complete rehydration. Using this method, a slightly uneven distribution of cells within the scaffolds is obtained.
The “Drop-in” cell seeding method will give a very efficient, deep, uniform and homogeneous distribution of cells during seeding. Therefore, rapid “dropping” or active immersion of the scaffolds into an excess volume of freshly mixed cell suspension is recommended. After a few seconds, complete rehydration and uniform cell seeding is achieved.
Cell culture conditions should meet the requirements of the cell type being used. During 3D cell culturing, high numbers of cells are cultivated in relatively small volumes. Therefore, it is recommended to provide excess medium at a high refreshment rate. OptiMaix scaffolds may be used in either static, dynamic or bioreactor based cell culture. Dynamic cell culture conditions may be beneficial for the differentiation of cells within the scaffolds.
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